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CV chercheurs

Yonghua Li-Beisson

​Chercheur CEA (PhD, HDR)

Publié le 28 juin 2018

Department of Plant Biology and Environmental Microbiology
CEA/CNRS/Aix-Marseille University, France

Tel: 33-442254651; Fax: 33-442256265; Email: yonghua.li@cea.fr

Lab home page: http://www-dsv.cea.fr/lb3m

ORCID: 0000-0003-1064-1816


2009-present: Senior Scientist. The French Alternative Energies and Atomic Energy Commission (CEA Cadarache), France

2008-2009: Associate Researcher. The French National Center for Scientific Reseach (CNRS), Bordeaux, France

2003-2008: Postdoc Fellow. The laboratory of Professor John Ohlrogge, Michigan State University, USA

2002-2003: Postdoc fellow. Department of Plant Biology, University of Oxford, England


Jan 2002: PhD thesis. The laboratory of Professor Colin Ratledge, University of Hull, England

Oct 2012: HDR (Habilitation à Diriger la Recherche), Aix Marseille University, France


Member of the Editorial Board:
  • Guest editor for: The Plant Cell (since 2016-) 
  • Handling editor for: Plant Cell and Physiology (since 2017-)
  • Reviewing editor for: mSphere (then Eukaryotic Cell)(since 2015-) 
  • Editorial board for: OA Biotechnology, Frontiers in Plant Metabolism and Chemodiversity (since 2013-) 
Member of the Scientific Organizing Committee for:
  • International Symposium on Plant Lipids (ISPL): Sevilla 2012, Toronto 2014, Goettingen 2016 
  • Euro Fed of Plant Lipids (Eurofed) 2015, 2017 
  • GERLI - The French Lipidomics Group 
Discussion leader for Gordon Conference "Plant Lipids: structure, metabolism and function", 2015

Expert for evaluation of: 
  • Research grants: University of Hong Kong; Israel national Grant; The Israel-US bilateral funds; NSF; ANR "Blanche", ANR "JCJC"; BBSRC; ERASynBiol; National Research Fundation of Korea 
  • Journals for Science, PNAS, Plant Cell, Plant J, JBC, Plant Physiology, etc
Senior Expert for the CEA "Haut Commissaire" since 2014


Lipid biology and biotechnology in green photosynthetic cells (microalgae and higher plants).
We focus on study of various aspects of lipid metabolism, including fatty acid synthesis, modification, turnover, their acylation to glycerolipids, the regulation of membrane lipid homeostasis and triacylglycerol synthesis and storage - the biogenesis, composition and function of lipid droplets. We explore how to make use of the knowledge generated to re-orient or create new pathways, for production of commercially desirable and lipid-derived compounds from microalgae. To this goal, we have developed and applied a range of tools including algal molecular genetics, high-throughput screening, lipidomics, proteomics, transcriptomics, imaging, and biochemical approaches.

Fatty acids and lipids are synthesized by all cell types. Besides their role as a major form of carbon and energy storage, fatty acids are basic building blocks of biological membranes, part of cellular signaling network, form protective outer envelopes. Research on lipids is both fundamental and applied. From a biotechnological point of view, lipids are essential part of our diet, source of chemical feedstocks, and a major player as a renewable fuel.

Oil is the most reduced form of energy found in nature, and represents twice more energy per gram dry biomass than other storage compounds (starch or protein). Oil (= triacylglycerols), as the name implies, is composed of three often different fatty acids which are esterified to the 3 hydroxyl groups of a glycerol backbone (Figure 1). Function and chemical properties of the oil are conferred largely by the structure of the fatty acids present. Many thousands of fatty acid structures occur in nature (PhyloFA: https://phylofadb.bch.msu.edu/). They differ in the total number of carbon, degree of unsaturation, with or without further fatty acid modification (for example, hydroxylation, epoxidation, dicarboxylation etc).

Figure 1: Structures of fatty acids and triacylglycerols

Current production of lipids to meet diet and industrial applications is far from sufficient. Novel sources with desirable product in large amount and easily extractable format are highly needed. A more specific problem to lipid production from microalgal strains is the observation that conditions favoring high oil accumulation often result in an arrest in cell growth and biomass production (Figure 2).

Figure 2: Cell growth, nitrogen status and oil accumulation

To solve some of these issues, we use the unicellular green microalga Chlamydomonas reinhardtii as a model organism (Figure 3) to answer some of these questions:

  1. How are lipids made and metabolized in algal cells ?
  2. What are the cellular events which underlie the trigger of oil accumulation ?
  3. What are the key enzymes and proteins involved in lipid turnover in algae ?
  4. What are the impact of subcellular coordination and energy trafficking on lipid metabolism ?
  5. What are the impact of alterations of lipid metabolism on cell physiology and fitness ?
Figure 3: Chlamydomonas reinhardtii as a model

An integrated overview of oil accumulation and degradation in microalgae​

As a first step toward understanding oil accumulation in Chlamydomonas reinhardtii, we followed the changes in cell morphology, chlorophyll, starch and lipid (membrane and storage) content over time. Upon removal of nitrogen from the media, the appearance of cellular oil droplet and starch granules is coincident with the disappearance of chlorophyll and thylakoid membranes. The stored oil are rapidly degraded upon nitrogen re-availability (Figure 4). The molecular mechanisms of the accumulation and degradation of oil are poorly understood, which is one of the current focus of our group. Results obtained down this line should yield important insights into the lipid homeostasis and therefore the overall fitness of cells.

Figure 4: Oil content of Chlamydomonas cells can be manipulated easily via changing N level

Lipid droplet - LD

Lipid droplet are subcellular compartment where neutral lipids are stored. It is ubiquitous in all eukaryotes. Other names for this cellular compartment are oil bodies or oleosomes. LDs are spherical organelles consisting of a neutral lipid core enclosed by a membrane lipid monolayer coated with proteins. Until fair recently, LDs are considered only as an energy and carbon storage site. Modern mass spectrometry has revealed the presence of many proteins in the isolated LD fraction. Well characterized structural proteins of oil droplet include oleosins found in oilseeds, or perilipin in adipocytes.

Using mass spectrometry on purified LDs from Chlamydomonas cells starved for nitrogen, our lab together with several other labs have identified a novel structural protein of algal lipid droplets – named Major Lipid Droplet Protein (MLDP). Besides this protein, numerous metabolic enzymes or lipid trafficking proteins are also present for example acyl activating enzymes, acyltransferases or lipases. The enzymes present span the key steps of the triacylglycerol synthesis pathway and including a glycerol-3-phosphate acyltransferase (GPAT), a lysophosphatidic acid acyltransferase (LPAT) and a putative phospholipid:diacylglycerol acyltransferase (PDAT) (Figure 5).

Figure 5: Lipid droplets are dynamic cellular structures and enriched in enzymes of lipid metabolism

Furthermore, we have more recently provided proteomic, lipidomic and microscopic evidence that cells accumulate different populations of LDs when subjected to high light exposure as compared to the well-studied N starvation (Figure 6). These work highlight the dynamic nature and the role of LDs in relation to the physiological adaptations of algal cells to their environment.

Figure 6: High light-induced LDs are rich in lipids of plastidial origin

Lipid droplets are now believed to be not only the storage compartments (sink) but also are dynamic structures (node) likely to be involved in processes such as oil synthesis, degradation and lipid homeostasis. Detailed characterization of these LD associated proteins should yield important insight into the compartmentalization and the function and biogenesis of LDs in algae.

Furthermore, lipid droplets are often found to be physically associated to other subcellular organelles such as chloroplast, peroxisome, or mitochondria. Understanding of the energy trafficking pathways, or metabolite shuttles between these organelles should have significant impact not only on the understanding of the subcellular organization of metabolic pathways and their controls, but also on cell physiology, fitness and production of energy dense molecules such as lipids.

Forward genetic screens

Most of our current knowledge on oil synthesis in algae is deduced from plant pathways based on comparative genomics or sequence homologies. Few proteins of lipid metabolisms have been characterizes so far. To reveal novel players of oil metabolism in algae, we have set up two forward genetic screens (Figure 7). Chlamydomonas is a unicellular microalga and most of its life cycle stays as haploid. Generation of mutants is therefore a very powerful approach because the mutant phenotype can be seen in the first generation. Several methods of high throughput screening have been set up (Nile Red neutral lipid staining coupled with flow cytometer, cell counter and HP-TLC; direct transmethylation and GC-FID). Screening of mutants with one of the following phenotypes have been carried out:

  1. Mutants that show altered fatty acid composition
  2. Mutants that store more or less cellular oil than its progenitor during optimal growth
  3. Mutants that can store more or less oil under nitrogen starvation
  4. Mutants that were defected in oil remobilization following a period of N starvation
Figure 7: Forward genetic screens of lipid mutants

Four sets of mutants have been isolated: and some are shown to be altered in the fatty acid composition, some can accumulate significant amount of oil during optimal growth (Figure 8), and we have very lately shown that strains with an attenuated β-oxidation cycle accumulated more oil than WT (Figure 9).

Figure 8: Isolation of a mutant which accumulates neutral lipid under non-stress conditions

Figure 9: Shutting down fatty acid β-oxidation increases oil content

Genetic engineering in algae

We have in the meantime, initiated some work on the engineering of oil content or composition in Chlamydomonas through gene expression in the plastid genome. In collaboration with the laboratory of Professor Youngsook Lee (Postech, South Korea), we have recently shown that cellular oil content can be increased by 20% when a plastidial LPAT is overexpressed in the plastid genome (Figure 10).

Figure 10: Metabolic engineering of lipid metabolism in Chlamydomonas through gene expression in the chloroplast

PUBLICATIONS (peer-reviewed)​

(* corresponding author)
Published under either the name of Li or Li-Beisson
ORCID: 0000-0003-1064-1816


  1. Kong F, Liang Y, Légeret B, Beyly-Adriano A, Blangy S, Haslam R, Napier J, Beisson F, Peltier G, Li-Beisson Y* (2017) Chlamydomonas carries out fatty acid β-oxidation in ancestral peroxisomes using a bota fide acyl-CoA oxidase. The Plant J. in press
  2. Lee EJ, Oh M, Hwang JU, Li-Beisson Y, Nishida I, Lee Y (2017). Seed-specific overexpression of the pyruvate transporter BASS2 increased oil content in Arabidopsis seeds. Frontiers in Plant Science in press
  3. [2016]

  4. Légeret B, Schulz-Raffelt M, Nguyen HM, Auroy P, Beisson F, Peltier G, Blanc G, Li-Beisson Y* (2016) Lipidomic and transcriptomic analyses of Chlamydomonas reinhardtii under heat stress unveil a direct route for the conversion of membrane lipids into storage lipids. Plant, Cell & Environment 39:834
  5. Schulz-Raffelt M, Chochois V, Auroy P, Cuiné S, Billon E, Dauvillée D, Li-Beisson Y, Peltier G (2016) Hyper-accumulation of starch and oil in a Chlamydomonas mutant affected in a plant-specific DYRK kinase. Biotechnology for Biofuels 9:1
  6. Li N, Xu C, Li-Beisson Y, Philippar K (2016) Fatty acid and lipid transport in plant cells. Trends in Plant Science 21: 145 (invited review)
  7. Yamaoka Y, Achard D, Jang S, Legeret B, Kamisuki S, Ko D, Schulz-Raffelt M, Kim Y, Song WY, Nishida I, Li-Beisson Y*, Lee Y* (2016) Identification of a Chlamydomonas plastidial 2-lysophosphatidic acid acyltransferase and its use to engineer microalgae with increased oil content. Plant Biotechnology Journal 14:2158
  8. Li-Beisson Y, Verdier G., Xu L, Beisson F. (2016) Cutin and suberin polyesters. In Encyclopedia of life science eLS John Wiley & Sons, Ltd
  9. Yang WL, Pollard M, Li-Beisson Y, Ohlrogge J (2016) Quantitative analysis of glycerol in Arabidopsis dicarboxylic acid-rich cutins provides new insights into cutin structure. Phytochemistry 130:159
  10. Goold HD, Nguyen HM, Kong F, Beyly-Adriano A, Legeret B, Billon E, Cuine S, Beisson F, Peltier G, Li-Beisson Y* (2016) Whole genome re-sequencing identifies a quantitative trait locus repressing carbon reserve accumulation during optimal growth in Chlamydomonas reinhardtii. Scientific Report 6: 25209
  11. Li-Beisson Y*, Nakamura Y, Harwood J (2016) Lipids: from chemical structures, biosynthesis, and analyses to industrial applications. Subcellular Biochemistry 86: 1-18
  12. Goold HD, Cuiné S, Légeret B , Liang Y, Brugière S, Auroy P, Javot H, Tardif M, Jones BJ, Beisson F, Peltier G, Li-Beisson Y* (2016) Saturating light induces sustained accumulation of oil primarily stored in lipid droplets of plastidial origin in Chlamydomonas reinhardtii. Plant Physiology 171:2406.
  13. Sorigué D, Légeret B, Cuiné S, Morales P, Mirabella B, Guedeney G, Li-Beisson Y, Jetter R, Peltier G, Beisson F (2016) Microalgae synthesize hydrocarbons from long-chain fatty acids via a light-dependent pathway. Plant Physiology 171:2393
  14. [2015]

  15. Li-Beisson Y*, Beisson F, Riekhof W (2015) Metabolism of acyl-lipids in Chlamydomonas reinhardtii. Plant Journal 82:504 (invited review)
  16. Kim H, Jang S, Kim S, Yamaoka Y, Hong D, Song WY, Nishida I, Li-Beisson Y, Lee Y (2015) The small molecule fenpropimorph rapidly converts chloroplast membrane lipids to triacylglycerols in Chlamydomonas reinhardtii. Frontiers in Microbiology 6 :54
  17. Taleb A, Pruvost J, Legrand J, Marec H, Le-Gouic B, Mirabella B, Legeret B, Bouvet S, Peltier G, Li-Beisson Y, Taha S, Takache H (2015) Development and validation of a screening procedure of microalgae for biodiesel production: Application to the genus of marine microalgae Nannochloropsis. Bioresource Technology, 177:224
  18. [2014]

  19. Goold H, Beisson F, Peltier G, Li-Beisson Y* (2014) Microalgal lipid droplets: composition, diversity, biogenesis and functions. Plant Cell Reports 34 :545 (invited review)
  20. Li-Beisson Y, Peltier G, Knörzer P, Happe T, and Hemschemeier A (2014) Hydrogen and biofuel production in the chloroplast, in the book Plastid Biology edited by Theg SM and Wollman FA.
  21. [2013]

  22. Cagnon C, Mirabella B, Nguyen HM, Beyly-Adriano A, Bouvet S, Cuiné S, Beisson F, Peltier G, Li-Beisson Y* (2013) Development of a forward genetic screen to isolate oil mutants in the green microalga Chlamydomonas reinhardtii. Biotechnology for Biofuels 6:178
  23. Li-Beisson Y*, Shorrosh B, Beisson F, Andersson M, et al. (2013) Acyl-lipid metabolism: in The Arabidopsis Book, Rockville, MD: American Society of Plant Biologists 11:e0161
  24. Kim S, Kim H, Ko D, Yamaoka Y, Otsuru M, Kawai-Yamada M, Ishikawa T, Oh HM, Nishida I, Li-Beisson Y, Lee Y (2013) Rapid induction of lipid droplets in Chlamydomonas reinhardtii and Chlorella vulgaris by Brefeldin A. Plos One 8:e81978
  25. Nguyen HM, Cuiné S, Beyly-Adriano A, Légeret B, Billon E, Auroy P, Beisson F, Peltier G, Li-Beisson Y* (2013) The green microalga Chlamydomonas reinhardtii has a single ω-3 fatty acid desaturase which localizes to the chloroplast and impacts both plastidic and extraplastidic membrane lipids. Plant Physiol. 163:914
  26. Li-Beisson Y* and Peltier G (2013). Third-generation biofuels: current and future research on microalgal lipid biotechnology OCL (Oléagineux, Corps Gras Lipides) 20(6) D606 (invited review)
  27. Delrue F, Li-Beisson Y, Setier P-A, Sahut C, Roubaud A, Froment A-K, Peltier G (2013) Comparison of various microalgae liquid biofuel production pathways based on energetic, economic and environmental criteria. Bioresource technology 136:205
  28. [2012]

  29. Beisson F, Li-Beisson Y and Pollard M (2012). Solving the puzzles of cutin and suberin polymer biosynthesis. Current Opinion in Plant Biology 15:329 (invited review)
  30. Li-Beisson Y (2012). Triacylglycerol biosynthesis in eukaryotic microalgae - the biological basis for a sustainable 3rd generation biofuel. Lipid library, American Society of Oil Chemist (AOCS), edited by William Bill Christie (invited review)
  31. Yang W, Simpson JP, Li-Beisson Y, Beisson F, Pollard M, Ohlrogge JB (2012) A land-plant-specific glycerol-3-phosphate acyltransferase family in Arabidopsis: substrate specificity, sn-2 preference, and evolution. Plant Physiology 160(2):638:52


  1. Nguyen HM, Baudet M, Cuiné S, Adriano JM, Barthe D, Billon E, Bruley C, Beisson F, Peltier G, Ferro M, Li-Beisson Y* (2011). Proteomic profiling of oil-bodies isolated from the unicellular green microalga Chlamydomonas reinhardtii: with focus on proteins involved in lipid metabolism. Proteomics 
  2. Siaut M, Cunié S, Cagnon C, Fessler B, Nguyen M, Carrier P, Beyly A, Beisson F, Triantaphylidès C, Li-Beisson Y* and Peltier G (2011), Oil accumulation in the model green alga Chlamydomonas reinhardtii: characterization, variability between common laboratory strains and relationship with starch reserves. BMC Biotechnology , 11:7 doi:10.1186/1472-6750-11-7
  3. Li-Beisson Y (2011). Cutin and Suberin. In: Encyclopedia of Life Sciences (ELS), John Wiley & Sons, Ltd: Chichester Doi: 10.1002/9780470015902.a0001920.pub2 (invited review)


  1. Li-Beisson Y*, Shorrosh B, Beisson F, Andersson M, et al. (2010) Acyl Lipid Metabolism: in The Arabidopsis Book, Rockville, MD: American Society of Plant Biologists doi: 10.1199/tab.0133
  2. Manas-Fernandez A, Li-Beisson Y, Alonso, DL and Garcia-Maroto F (2010). Cloning and molecular characterization of a glycerol-3-phosphate Oacyltransferase (GPAT) gene from Echium (Boraginaceae) involved in the biosynthesis of cutin polyesters. Planta 232:987.
  3. Yang W, Pollard M, Li-Beisson Y, Beisson F, Feig M, Ohlrogge J (2010) A distinct type of glycerol-3-phosphate acyltransferase with sn-2 preference and phosphatase activity producing 2-monoacylglycerol. Proc Natl Acad Sci USA 107:12040
  4. Stork J, HarrisD, WilliamsB, Griffiths J, HaughnG, BeissonF, Li-Beisson Y, MenduV, DeBolt S (2010) CELLULOSE SYNTHASE9 serves a non-redundant role in secondary cell wall synthesis in the radial wall of Arabidopsis epidermal testa cells. Plant Physiology 153:580


  1. Li-Beisson Y, Pollard M, Sauveplane V, Pinot F, Ohlrogge J and Beisson F (2009), Nanoridges that characterize the surface morphology of flowers require the synthesis of cutin polyester. Proc Natl Acad Sci USA, 106:22008
  2. Molina I, Li-Beisson Y, Beisson F, Ohlrogge J and Pollard M (2009) Identification of an Arabidopsis feruloyl-CoA transferase required for suberin synthesis. Plant Physiol 151:1317
  3. DeBolt S, Scheible W, Schrick K, Auer M, Beisson F, Bischoff V, Bouvier-Navé P, Carroll A, Hematy K, Li Y, Milne J, Nair M, Schaller H, Zemla M and Somerville C (2009) Mutations in UDP-glucose:sterol-glucosyltransferase in Arabidopsis cause transparent testa phenotype and suberization defect in seeds. Plant Physiol 151:78
  4. Li Y* and Beisson F (2009). The biosynthesis of cutin and suberin as an alternative source of enzymes for the production of bio-based chemicals and materials. Biochimie 91: 685 )


  1. Pollard M, Beisson F, Li Y and Ohlrogge J (2008) Building lipid barriers: biosynthesis of cutin and suberin. Trends in Plant Sciences 13:236. (review)
  2. Li Y, Beisson F, Koo A, Molina I, Pollard M and Ohlrogge J (2007) Identification of acyltransferases required for cutin biosynthesis and production of cutin with suberin-like monomers. Proc Natl Acad Sci USA 104:18339
  3. Li Y, Beisson F, Ohlrogge J and Pollard M (2007) Monoacylglycerols are components of root waxes and can be produced in the aerial cuticle by ectopic expression of a suberin-associated acyltransferase. Plant Physiol 144:1267
  4. Beisson F*, Li Y*, Bonaventure G, Pollard M and Ohlrogge J (2007) The acyltransferase GPAT5 is required for synthesis of suberin in seed coat and root of Arabidopsis. Plant Cell 19:1 (*co-first authors)
  5. Li Y, Beisson F, Pollard M and Ohlrogge J (2006) Oil content of Arabidopsis seeds: the influence of seed anatomy, light and plant-to-plant variation. Phytochemistry 67:904
  6. Li Y, Adams IP, Wynn JP and Ratledge C (2005) Cloning and characterisation of a gene encoding a malic enzyme involved in anaerobic growth in Mc circinelloides. Mycological Research 109:461
  7. Li Y, Challen M, Elliott T and Casselton L (2004) Molecular analysis of breeding behaviour in Agaricus species. Mushroom Science 16:103
  8. Song Y, Wynn JP, Li Y, Granham D and Ratledge C (2001) A pre-genetic study of the isoforms of malic enzyme associated with lipid accumulation in Mucor circinelloides. Microbiology 147:1507
  9. Wynn JP, Hamid AA, Li Y and Ratledge C (2001) Biochemical events leading to the diversion of carbon into storage lipids in the oleaginous fungi Mucor circinelloides and Mortierella alpine. Microbiology 147:2857​

OTHER (not peer-reviewed publications)

  1. Beisson F, Li-Beisson Y, Peltier G, Finazzi G, Maréchal E, Chauvat F, Delrue F, Froment AK, Blet V (2012). Des microalgues pour la production de biocarburants. Clefs CEA
  2. Li-Beisson Y and Peltier G (2011). Biocarburants: le défi des microalgues. Pour la Science issue 78 (in French) (invited contribution)
  3. Editor for the book “Lipids in Plant and Algae Development” ‘(Nakamura Y and Li-Beisson Y, eds., Springers 2016).


  1. Schulz-Raffelt M, Chochois V, Li-Beisson Y, Peltier G (2014) Green microalgae lacking a functional DYRK-1 gene, for use for production of feedstock (7/05/2014 EP14305673.7).
  2. Ohlrogge J, Beisson F, Li Y, Pollard M (Jan 15 2007). Engineered plant extracellular lipids using acyltransferases and fatty acid omega-oxidases (PCT/US2008/067887).
  3. Li Y, Beisson F, Pollard M and Ohlrogge J (Jun 22 2006). Acyltransferases for altering lipid production on plants (PCT/US2007/014690).
  4. Ohlrogge J, Ruuska S, Li Y (March 29 2011). F-Box protein targeted plant oil production. United States Patent 7,915,480 (PCT/US2006/037111).


​​Aralip (Arabidopsis Lipid Metabolism database):http://arabidopsisacyllipids.plantbiology.msu.edu/pathways


​​​Invited presentations (since 2009-):

  1. Microalgae biofuel: from genomics, genetics to energy dense molecules. The 9th Asia-Pacific Conference on Algal Biotechnology (APCAB), Bangkok, Thailand (2016)
  2. Advances in biosynthesis and degradation of lipids in microalgae. 22nd International Symposium on Plant Lipids (ISPL), Germany (2016)
  3. Study of oil turnover in the model green microalga Chlamydomonas reinhardtii. 7th European Symposium on Plant Lipids, U.K. (2015)
  4. Dissecting oil metabolism in Chlamydomonas using genetic and lipidomic approaches. 3rd European Symposium on Microbial Lipids, Germany (2014)
  5. Lipid metabolism in the green microalga Chlamydomonas reinhardtii: current research and future challenges. The French Photosynthetic Society Meeting, France (2013)
  6. Orientation of cellular metabolism toward oil production in microalgae: stress and genetics. The 9th Plasticity and Integrity of Genomes Conference, France (2013)
  7. Genetic and lipidomic approaches in deciphering lipid metabolism in Chlamydomonas. 3rd Gordon Conference (Plant Lipids: Structure, Metabolism & Function), USA (2013)
  8. Genetic approaches to unravel lipid metabolism in the model alga Chlamydomonas reinhardtii. Plant Lipid Metabolic Network and Switching, Japan (2013)
  9. Screens, mutants, and –omics approaches toward understanding of lipid metabolic pathways in microalgae. 5th Asian Symposium on Plant Lipids, South Korea (2013)
  10. Deciphering TAG accumulation in Chlamydomonas using a proteomic approach. 15th International Conference on Chlamydomonas, Germany (2012)
  11. Unravelling the networks of oil metabolism in green microalgae using Chlamydomonas reinhardtii as a model. CEA/DSV Bioenergy Conference, France (2012)
  12. Current approaches to unravel oil biosynthesis in microalgae using Chlamydomonas reinhardtii as a model. NREL-CEA joint meeting, USA (2012)
  13. Biofuel from microalgae - current and future challenges for research. Nouvelle Technologies pour le Energies (NTE-DRT), France (2012)
  14. A forward genetic approach toward unravelling factors critical for oil metabolism in the model green alga Chlamydomonas reinhardtii. 20th International Symposium on Plant Lipids (ISPL), Spain (2012)
  15. Lipid biosynthesis in Chlamydomonas reinhardtii. 4th Asian Symposium on Plant Lipids, Hong Kong (2011)
  16. Oil synthesis in the green microalga Chlamydomonas reinhardtii – a proteomic approach. 5th European Symposium on Plant Lipids, Poland (2011)
  17. Polyester Biosynthesis as a Source of Fatty Acid Hydroxylases and Acyltransferases with New Substrate Specificities. 19th International Symposium on Plant Lipids (ISPL) Carines, Australia (2010)
  18. Biosynthesis and function of plant cuticular polyesters. 1st Gordon Conference (Plant Lipids: Structure, Metabolism & Function), USA (2009)

Invited departmental seminars:

  1. Re-orientation of cellular metabolism toward oil synthesis in green microalgae: stress and genetics. Centre for Algal Research, Beijing, China (2016)
  2. Current research on algae biofuel at CEA Cadarache. Institute of Hydrobiology, Chinese Academy of Science, Wuhan, China (2016)
  3. Microalgae biofuel: from genomics, genetics to energy dense molecules. University of Kaiserslautern, Germany (2016)
  4. Current research on lipids in CEA Cadarache -With focus on forward genetic approaches. University Paris Sud, Orsay, France (2015)
  5. Biocarburants à partir de micro-algues: quels enjeux pour la recherche? Marseille, Faculté de pharmacie 2015
  6. Lipid metabolism in the model microalga Chlamydomonas reinhardtii. Georg-August-Universität Göttingen, Goettingen, Germany (2015)
  7. Lipid biosynthesis and homeostasis in the model microalga Chlamydomonas reinhardtii - With focus on forward genetic approach. Opening ceremony for Centre for Lipid Research, Zibo, China (2015)
  8. Lipid biosynthesis and homeostasis in the model green microalga Chlamydomonas reinhardtii. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Science, Tianjin, China (2015)
  9. Screens, mutants, and –omics approaches toward understanding lipid metabolic pathways in the alga Chlamydomonas reinhardtii. Ludwig Maximillian’s University, Germany (2014)
  10. Lipid research on the 3rd generation biofuel from microalgae at CEA Cadarache. South West University, China (2014)
  11. A forward genetic screen to isolate oil mutants in the green microagla Chlamydomonas reinhardtii, current research and future challenges, POSTECH, South Korea (2013)
  12. Current lipid research at CEA Cadarache France. Shizuoka University, Shizuoka, Japan (2013)
  13. Lipid Metabolism in Microalgae- Current progress and future directions. Martin Luther University Halle-Wittenberg, Germany (2012)
  14. Unrevalling lipid metabolism in green microalgae using Chlamydomonas reinhardtii as a model. POSTECH, South Korea (2011)
  15. Lipid metabolism in the green model microalga Chlamydomonas reinhardtii. Chonnam National University, Chonnam, South Korea (2011)
  16. The molecular analysis of breeding behaviour of Agaricus bisporus.Department of Plant Sciences, University of Oxford, UK (2003)
  17. The initial events leading to onset of oil biogenesis in fungi. Department of Biological Sciences, University of Hull, UK (2001)
  18. Oil biosynthesis in oleaginous fungi Mucor circinelloides. Zhengzhou Institute of Technology, China (2000)

Contributed talks at international meetings:

  1. Dissecting oil metabolism in Chlamydomonas using genetic and lipidomic approaches. 3rd European Symposium on Microbial Lipids, Hamburg (2014)
  2. Deciphering triacylglycerol accumulation in Chlamydomonas reinhardtii using a proteomic approach. 15th International Conference on the Cell and Molecular Biology of Chlamydomonas, Germany (2012)
  3. Oil synthesis in the green microalga Chlamydomonas reinhardtii – a proteomic approach. 5th European Symposium on Plant Lipids, Poland (2011)
  4. A member of an orphan family of cytochrome P450 monooxygenases is required for the synthesis of hydroxy fatty acids in plants. 5th Lipidomics Meeting (GERLI), France (2008)
  5. Glycerol 3-phosphate acyltransferase 5 is involved in suberin synthesis. Plant Research Lab-DOE, Michigan State University, USA (2007)
  6. Characterization of an acyltransferase mutant of Arabidopsis with altered seed coat lipid metabolism. National Plant Lipid Cooperative Meeting, USA (2005)
  7. Initiation of lipid biosynthesis in oleaginous fungi. “New Concepts in Lipid Research” national meeting of SCI/RSC Lipid Group, London, UK (2001)


MUsCA (2014-2018) – « Metabolic engineering of a green microalga for production of medium-chain alkanes»
Coordinator : Yonghua Li-Beisson (CEA Cadarache)
ANR Programme JCJC (French National Grant for Young Scientists)

SignauxBioNRJ (2016-2020) - «Manipulating energy signaling to improve biofuel production in photosynthetic eukaryotes »
Coordinator: Benoit Menand, Ben Field (CNRS, Marseille)
ANR Programme Bioenergies

DIESALG (2012-2015) – « Biodiesel production by microalgae»
Coordinator : Jérémy Pruvost (GEPEA, Nantes)
ANR Programme Bio-matières et Energies

NannoControl (2012-2015) “Development of molecular tools for the control of the expression of genes (knockout of native genes, constitutive or promoter-dependent expression of transgenes) in Nannochloropsis species”
Coordinator: Yonghua Li-Beisson (iBEB), Giovanni Finazzi (iRTSV/LPCV)
Programme Interne du CEA


Teach « Lipid Metabolism and Lipid Biotechnology » 4 h per year: in the module « Plants, Energy and Light » for Master level at the University of Aix Marseille, France.